130 The Effect of Serum from Preeclampsia Mother and Her Neonatal Cord Blood on The Activity of Soluble Adhesion Molecules and Apo-Ptosis in Cultured Huvec

Abstract

Purpose: In this study we tested the effect of serum from preeclampsia mother and her neonatal umbilical cord bood on the activity of soluble intercellular adhesion molecule-1(sICAM-1) and apoptosis in cultured human umbilical venous endothelial cells(HUVEC). We also determined if unknown circulating factors in preeclamptic women may influence to the endothelial cells of her fetus. Study design: Maternal serum samples were collected before delivery from women with preterm and term preeclampsia (preeclamptic term mother group, n= 14, preeclamptic preterm mother group, n= 12) and uncomplicated control pregnancies (control term mother group, n= 12, control preterm mother group, n= 11). Neonatal serum samples of these women were also collected from the umbilical veins(preeclamptic term cord group, n= 13, preeclamptic preterm cord group, n= 12, control term cord group, n= 12, control preterm cord group, n= 11). The sICAM-1 were measured with a sandwich ELISA technique in isolated Results: 1) In sICAM-1 levels: Each mother groups determined significantly higher levels compared to each neonatal cord groups(p< 0.001). Preeclamptic mother groups showed increased levels compaired to control mother groups but have no statistical differences(p0.05). However, the preterm mother groups determined increased levels compaired to term mother groups(p< 0.001). In between each mother groups, preterm preeclampsia mother groups were significantly elevated compared to other mother groups(p< 0.001). In between neonatal cord groups, there were no statistical differences in each groups. 2) In TUNEL stain: Preterm preeclampsia mother group showed significantly increased number of apoptotic nuclei and decreased number of cells compaired to other groups. Especially, the neonatal cord groups of preterm preeclampsia showed no apoptotic body on cultured HUVEC. Conclusions: Unknown circulating factors in preterm preeclampsia mother activate expression of ICAM-1 and apoptosis in cultured human umbilical venous endothelial cells. But the fetal circulation may not be affected by the factor(s) that lead to disturbed endothelial cell function in women with preterm preeclampsia.