130 Antimicrobial activity of liposomal β-glycan against Pseudomonas aeruginosa isolated from cystic fibrosis patients

Abstract

Objective Management of pseudomonal pulmonary infections associated with cystic fibrosis is often difficult due to the development of resistance to commonly used antibiotics. Our objective was to find new therapeutic strategies to treat drug resistant infection by the use of liposomal formulations. Methods The antimicrobial activity of free and liposomal b-glycan against Pseudomonas aeruginosa strains isolated from cystic fibrosis patients were examined. The bacterial motility, attachment, and virulence factors in the presence of these liposomal formulations were also evaluated. The release kinetics in bronchoalveolar lavage was assessed by microbiological assay. Results A strong antibacterial activity against most of the P. aeruginosa strains was observed in the presence of liposomal b-glycan compared to free b-glycan. Liposomal b-glycan at sub-inhibitory concentrations, attenuated the P. aeruginosa twitching swarming and swimming on agar, reduced bacterial adhesion and viability in 96-well plates and decreased protease and elastase levels. Conclusion These liposomal b-glycan formulations could be used in the management of pulmonary infections due to Pseudomonas aeruginosa in cystic fibrosis patients.