Abstract
Publisher Summary DNA gyrases are members of the Topo IIA family of type II DNA topoisomerases. They are unique among these enzymes in their ability to introduce negative supercoiling in a covalently closed circular DNA at the expense of ATP. DNA gyrase is a heterotetramer composed of two subunits, GyrA and GyrB. This chapter describes the purification of DNA gyrase from T. maritima , a hyperthermophilic anaeorobic bacterium from the order of Thermotogales , with an optimal growth temperature of 80°. This hyperthermophile contains both a DNA gyrase and a reverse gyrase. Also, the plasmid pRQ7, from another Thermotoga species, has been found to be negatively supercoiled. This indicates that when the two enzymes are present in the same organism, DNA gyrase activity predominates over that of the reverse gyrase in vivo .
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