Abstract
UNLIKE proton nuclear magnetic resonance (NMR) spectroscopy, 13C NMR has the potential advantages in studies of macromolecular systems of a wider range of chemical shifts (∼200 p.p.m.) and of reduced resonance broadening in structures with restricted molecular motion. Thus we have been able to observe 13C NMR spectra of unsonicated lecithin and erythrocytes, which do not yield high resolution proton spectra. We also report T1 relaxation times for 13C nuclei in the polar headgroup of the lecithin molecule and at both ends of the alkyl chains, in sonicated vesicles in D2O buffer and in deuterochloroform. The results are consistent with increasing chain motion towards the terminal methyl group of the alkyl chains for both systems, in agreement with relaxation data for stearic acid derivatives specifically labelled with 19F nuclei in lecithin vesicles1, and with the results of similar spin label experiments of Hubbell and McConnell2.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.