13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids.

Meng Zhang,Paula Jouhten,Gaetano T Montelione,Xiao‐Wei Yu,John F Hunt,Hannu Maaheimo,Ralf W Glaser,Yan Xu,Thomas Szyperski,Gurla V T Swapna

doi:10.1111/febs.14180

13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids.

Meng Zhang, Paula Jouhten + Show 8 more

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https://doi.org/10.1111/febs.14180

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Journal: FEBS Journal	Publication Date: Aug 7, 2017
Citations: 10

Affiliation: State Key Laboratory of Food Science and Technology, European Molecular Biology Laboratory, Rutgers, The State University of New Jersey, Columbia University, VTT Technical Research Centre of Finland, Friedrich Schiller University Jena, University at Buffalo, State University of New York

#Aerobic Batch Cultures #Regulation Of Central Carbon Metabolism + Show 8 more

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Abstract

Acetohydroxy acid isomeroreductase (EC 1.1.1.86), branched-chain amino acid aminotransferase (BCAT, EC 2.6.1.42), fumarase (EC 4.2.1.2), malic enzyme (EC 1.1.1.39/1.1.1.40), phosphoenolpyruvate carboxykinase (EC 4.1.1.49), pyruvate carboxylase (EC 6.4.1.1), pyruvate kinase (EC 2.7.1.40), l-serine hydroxymethyltransferase (EC 2.1.2.1), threonine aldolase (EC 4.1.2.5), threonine dehydratase (EC 4.3.1.19); transketolase (EC 2.2.1.1), transaldolase (EC 2.2.1.2).

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