12R-lipoxygenase activity is reduced in photodamaged facial stratum corneum. A novel activity assay indicates a key function in corneocyte maturation.

Abstract

BackgroundDuring the late stage of keratinocyte differentiation, corneocytes gain a strong protein–lipid structure: the corneocyte envelopes (CE), composed of the inner corneocyte protein envelope (CPE) and the outer corneocyte lipid envelope (CLE). The hydrophobicity of CEs depends on the covalent attachment of linoleoyl‐acyl‐ceramides by transglutaminases (TG). These ceramides are processed by a range of other enzymes, including 12R‐lipoxygenase (12R‐LOX), before the covalent attachment of the free ω‐hydroxyceramides to the CPE surface to form the CLE. The mechanical strength of CE is obtained with the formation of isodipeptide bonds by TG. The increase in hydrophobicity and rigidity leads to CE maturation which supports the integrity and mechanical resistance of the stratum corneum (SC).ObjectivesThe aim of this work was to develop and validate a novel enzyme activity assay for 12R‐LOX in tape strippings of photo‐exposed (PE) cheek and photo‐protected (PP) post‐auricular SC of healthy Chinese volunteers (n = 12; age 25 ± 3 years).ResultsA fluorescence‐based assay was developed with ethyl linoleic acid as the substrate and a polyclonal antibody against 12R‐LOX as an inhibitor. The specificity was shown by the lack of effect by a LOX inhibitor (ML351) and an epidermal‐type lipoxygenase 3 (eLOX3) antibody on the acquired 12R‐LOX activity. Reduced 12R‐LOX activity was observed in the outer compared to the inner SC layers. Moreover, dramatically lower activity was shown in the PE vs. PP samples. Furthermore, the enzyme activity has a positive correlation (r = 0.94 ± 0.03) with CE maturity, in particular hydrophobicity, and a negative correlation (r = −0.96 ± 0.01) with transepidermal water loss (TEWL).ConclusionThis novel enzyme assay revealed a lower 12R‐LOX activity in tape strippings from PE cheek for the first time. This finding is in line with less mature CEs and higher TEWL compared to PP post‐auricular samples. This study indicates a strong link between 12R‐LOX activity and CE maturation and SC integrity.