Abstract

Vitamin D3 is made in the skin through the photolysis of 7-dehydrocholesterol by UVB between 295-303 nm or obtained through food or supplements. Vitamin D3 does not have significant biological activity and must be converted to a biologically active form in a multistep reaction by either entering the blood stream where activation occurs in the liver then the kidneys or activation in the skin to be used locally. Interestingly, keratinocytes are the only cells in the body that can perform this process from start to finish. Active Vitamin D3 or calcitriol is important for proper skin function and 25-hydroxyvitamin D3 1-α hydroxylase (CYP27B1), a rate-limiting enzyme in the formation of active form of vitamin D3, was shown to be required for optimal epidermal differentiation and permeability barrier homeostasis. Therefore, identifying compounds that can increase expression of 25-hydroxyvitamin D3 1-α hydroxylase in skin is a viable approach to develop cosmetic products aiming to increase synthesis of active form of Vitamin D3 in skin, which could lead to barrier homeostasis. Human keratinocytes were treated in vitro with a cosmetic technology. After 24 hours of treatment, these keratinocytes exhibited an 86% increase in 25-hydroxyvitamin D3 1-α hydroxylase expression (p<0.05), normalized to GAPDH, compared to the untreated control. This increase suggests that the cosmetic technology could help facilitate the formation of Vitamin D produced in the skin, which has implications in barrier improvement. In-house clinical study of immediate barrier shows that a formulation containing patented technology blend significantly improve skin’s barrier 15 minutes after a single application compared to baseline and untreated control, as shown via TEWL measurements. This further suggest the role of this cosmetic technology in supporting skin barrier homeostasis.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.