Abstract
Crude preparations of pig spleen cell membranes were obtained by differential centrifugation. 125I-NPY bound specifically to these membranes with a K D of 56±13 pM and B max of 44±4.0 fmols/mg protein. After treatment with 1% CHAPS and 10mM 2-mercaptoethanol in the presence of 2μM leupeptin, 2μM pepstatin A, 10μM phosphoramidon, 200μM PMSF and 0.1% bacitracin, followed by centrifugation at 100,000g a soluble preparation was obtained that contained a single population of specific 125I-NPY binding sites. The K D of the soluble receptor was significantly higher at 1.38±0.2 nM (P<0.01) but the B max of 59.6±6.6 fmols/mg protein was similar (N.S.). This is the first description of a method for obtaining NPY receptors in soluble form and should enable their purification and characterisation, though the low affinity of the soluble receptor may reflect disruption of the ligand binding site upon removal of the receptor from the membrane.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
