1,2,4-Triaminobenzene as a Fluorescent Probe for Intracellular pH Imaging and Point-of-Care Ammonia Sensing

Abstract

As one of the health indicators, intracellular pH plays important roles in many processes of cell functions. Abnormal pH changes would result in the occurrence of inflammation, cancer, and other diseases. Thus, it is of significant importance to develop effective techniques for sensitive detection of pH changes for the clinical diagnosis of various diseases related to cells. In this paper, 1,2,4-triaminobenzene hydrochloride was explored as an organic molecular fluorescent probe for sensitive and selective detection of intracellular pH changes for the first time. Due to the protonation and deprotonation of amino groups of the probe, its fluorescent intensity at 599 nm or the ratio of absorbance at 505 and 442 nm has a good linear relationship with pH values in the range of 5.0-7.0. Benefiting from the excellent physical and chemical properties of 1,2,4-triaminobenzene hydrochloride, the fluorescent probe has good water solubility, low toxicity, high photostability, great reversibility, good cell penetration, fast response speed, and so on. As a proof-of-concept demonstration, the proposed probe is employed for the fluorescence imaging of cells and mouse tissue sections with satisfactory performance in pH differentiation. Additionally, the probe was successfully employed to prepare test strips as a kind of point-of-care testing device to detect ammonia, which showed great potential in practical applications.