1242. Quantitative Analysis of Microbial Burden on LTCF Environmental Surfaces

Abstract

BackgroundThere is a lack of data on environmental surface contamination in long-term care facilities (LTCF), despite multiple reports of outbreaks of multi-drug-resistant organisms in these settings. Therefore, we conducted a quantitative analysis of the microbial burden and prevalence of epidemiologically important pathogens (EIP) found on LTCF environmental surfaces.MethodsMicrobiological samples were collected using Rodac plates from resident rooms and common areas in five LTCFs. At each facility, five samples from up to 10 different available environmental surfaces were collected from a room of a resident reported to be colonized with EIP, as well as from a room of a resident reported to be non-colonized. In addition, five samples from up to 10 different environmental surfaces were collected from two common areas in the facility. EIPs were defined as MRSA, VRE, C. difficile and multi-drug-resistant Gram negative bacilli. Data were analyzed for each environmental site sampled in a resident room or common area based on total bacterial colony forming units (CFU), mean CFU per Rodac, total EIP by site, and mean EIP counts per Rodac.ResultsThe below table summarizes total EIP recovered from environmental sites by reported EIP colonization status of the resident. Rooms of residents with reported colonization had much greater EIP counts per Rodac (8.32, 95% CI 8.05, 8.60) than rooms of non-colonized residents (0.78, 95% CI 0.70, 0.86). MRSA was the most common EIP recovered from Rodacs, followed by C. difficile. Very few EIPs were recovered from the common areas sampled at these LTCFs.ConclusionWe found varying levels of CFU and EIP on environmental sites at LTCFs. Colonization status of a resident was a strong predictor of higher levels of EIP being recovered from his/her room.Table: Total EIP Recovered From Environmental Sites in Residential RoomsSiteNumber of Rodac SamplesTotal EIP by SiteMean EIP Counts per RodacNumber of Rodac SamplesTotal EIP by SiteMean EIP Counts per RodacNon-Colonized Resident RoomsColonized Resident RoomsBathroom Floor54350.6555182033.09Bed Rail48200.424561413.64Over Bed Table48240.50551232.24Nightstand5510.02492234.55Sink552514.56493717.57Chair3510.03443618.20Overall Sites Sampled4333370.7842835618.32Disclosures All authors: No reported disclosures.