Abstract
Introduction: As cancer treatment improves, more young women and girls survive. However, many suffer from premature ovarian failure as a consequence of anticancer therapy that promotes accelerated primordial follicle loss. Among the limited options available for fertility preservation is cryopreservation of ovarian tissue containing immature primordial follicles followed by autotransplantation. Although this procedure has so far resulted in seven live births to cancer survivors, there is increasing evidence that in various malignancies it also carries a risk of reseeding cancer. This drawback could be overcome by in vitro maturation of oocytes derived from primordial follicles followed by in vitro fertilization and embryo transfer. Therefore, improvements in culture techniques are urgently needed.
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