Abstract
Background: Photochemical tissue bonding (PTB) is a novel method for producing a strong tissue-tissue seal using photosensitization. PTB cross-links tissues without causing thermal damage, although the mechanism of action in skin is not completely understood. Apligraf, a bilayered tissue-engineered skin construct, is currently applied to chronic wounds and fixed in place by bolster, suture, staple, or glue. We postulated that PTB may have application for attaching Apligraf to skin. Materials and Methods: An ex vivo model was used to characterize bonding of Apligraf and skin by PTB. Argon laser (514 nm) or IPL irradiation in the presence of the photosensitizing dye Rose Bengal was used to bond Apligraf and human cadaveric or porcine skin. Various outcome measurements were made to evaluate the efficacy and histological characteristics of the bonding. Conclusions: Photochemical tissue bonding of Apligraf to human skin can be performed and further characterization is under way. Potential applications of PTB and Apligraf include the treatment of acute wounds, split thickness skin graft donor sites, burns, and debrided chronic wounds.
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