1207 AEROSOL TRANSFER OF BLADDER UROTHELIAL AND SMOOTH

Abstract

You have accessJournal of UrologyPediatrics: Reconstructive Surgery - Diversion, Endourology & Transplantation, Trauma1 Apr 20121207 AEROSOL TRANSFER OF BLADDER UROTHELIAL AND SMOOTH Guy Hidas, Hak Lee, Andrej Bahoric, Shuman Liu, Zhongbo Liu, Xiaolin Zi, Samah Saharti, David kerbl, Hung Truong, and Antoine. E. Khoury Guy HidasGuy Hidas Orange, CA More articles by this author , Hak LeeHak Lee Orange, CA More articles by this author , Andrej BahoricAndrej Bahoric Orange, CA More articles by this author , Shuman LiuShuman Liu Orange, CA More articles by this author , Zhongbo LiuZhongbo Liu Orange, CA More articles by this author , Xiaolin ZiXiaolin Zi Orange, CA More articles by this author , Samah SahartiSamah Saharti Orange, CA More articles by this author , David kerblDavid kerbl Orange, CA More articles by this author , Hung TruongHung Truong Orange, CA More articles by this author , and Antoine. E. KhouryAntoine. E. Khoury Orange, CA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1474AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In order to prevent the absorptive and secretory complications of the intestinal segments used for augmention, we have previously described a cell transfer technology for rapid coverage of demucosalized colonic segments, with an autologus mixture of cell suspensions urothelial, and detrusor smooth muscle cells combined with fibrin glue, sprayed over a demucosalized colon. In the initial study we reported on the histology and size of the segments at six weeks. In this study we maintained the animals for six months to confirm durability of the technology and to perform functional evaluation of the urothelia lined constructs. METHODS Nine pigs were divided into 3 groups. Group 1: Control group underwent colocystoplasty, group 2 and 3: underwent colonic demucosalization with aerosol application of fibrin glue with urothelial cells only (group 2) and urethelial with smooth muscle cells on the colonic segment (Group 3). Animals were kept for six months. Absorptive function was assessed by ligating the two ureters and measuring the volume of preinstalled 300ml distilled water for one hour in the bladder. Secretory function was assessed by measuring the total urine sediment after vigorous centrifugation as well as total urine protein content (measured by Lowri protein assay). Finally, the pigs were sacrificed and we measured the intestinal segment surface area and performed histological analysis. RESULTS The surface area of the augmented segments showed an increase in the control group 1, and was unchanged in groups 2 and 3. On H&E staining all group 1 animals showed normal colonic epithelium of the augment. All animals in groups 2 and 3 showed confluent urothelial lining with no fibrosis or inflammatory changes. Group 3 animals appeared to have a healthier urothelium with detrusor muscles deep to it. There was no evidence of colonic epithelial re-growth in any animal in groups 2 and 3. 10% of distilled water installed in the bladder was absorbed in the control group and non in group 2 and 3. Total urine sediment and protein was significantly higher in the control group compare to group 2 and 3. CONCLUSIONS This long term study showed that aerosol transfer of bladder urothelial and smooth muscle cells onto the demucosalized intestinal segment for bladder augmentation is viable technique that offer a histologically durable, confluent urothelium, without colonic mucosa regrowth or sub-mucosal fibrosis. The absorptive and secretory complication of incorporation gastrointestinal segment in the urinary system were not observed. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e488 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Guy Hidas Orange, CA More articles by this author Hak Lee Orange, CA More articles by this author Andrej Bahoric Orange, CA More articles by this author Shuman Liu Orange, CA More articles by this author Zhongbo Liu Orange, CA More articles by this author Xiaolin Zi Orange, CA More articles by this author Samah Saharti Orange, CA More articles by this author David kerbl Orange, CA More articles by this author Hung Truong Orange, CA More articles by this author Antoine. E. Khoury Orange, CA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...