120 Protection against acute cold stress-induced impairment of skin integrity

Abstract

Exposure of skin to different climatic conditions is considered to be a main source of discomfort with a significant impact on daily life. Mainly cold and dry weather, as encountered in the winter season in countries far away from the equator, has been associated with these negative effects. Here we present clinical data showing reduced barrier strength and increased xerosis during the winter season in Belgium, which is considered to be a mild climate. We then developed an in vitro model that mimics the effects of extreme cold weather conditions on skin. 3D epidermal skin models were exposed to an intermittent flow of liquid nitrogen air. The acute cold stress induced a reduction of the Trans Epithelial Electrical Resistance (TEER), caused toxicity and increased the release of inflammatory markers IL1a and IL8 in the medium. This model was used to evaluate the protective effect of topically applied cosmetic formulations. A significant protective effect was measured as a reduction in cell death, lower levels of inflammation markers, and maintenance of barrier function after the cold stress. In conclusion we have illustrated that in vivo cold stress caused impaired skin integrity. By means of a novel in vitro assay, we have identified cosmetic formulations that protect against the cold stress induced adverse modifications.