Abstract
Cyclic nucleotide-gated (CNG) channels comprise a family of cation-selective channels that are cooperatively activated or inhibited by the binding of cGMP or cAMP. In vertebrate rod and cone photoreceptor cells and olfactory receptor neurons, these channels play a central role in sensory transduction pathways by controlling the flow of Na + and Ca 2+ into the cell in response to signal-mediated changes in intracellular cyclic nucleotide levels. The physiologic role of CNG channels in nonsensory cells, however, has yet to be determined. The CNG channels of rod photoreceptors and olfactory neurons have been most extensively studied at a molecular level. This chapter describes several procedures that have been used to purify the cGMP-gated channel from ROS membranes and reconstitute the channel into lipid vesicles for functional analysis. The application of these methods to the isolation of CNG channels from other cells systems is discussed.
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