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Abstract

Case Reports: Acute paraquat (PQ) poisoning can lead to acute lung injury (ALI), acute respiratory distress syndrome (ARDS), multiple organ dysfunction syndrome (MODS), and eventually to death. However, some patients die shortly after PQ poisoning, considering that PQ may correlate with heart injury at an early stage.Objective-To investigate the mechanism of PQ poisoning induced heart injury and observe the therapeutic effects of ulinastatin on PQ poisoning induced rat heart injury. Methods-Established PQ poisoning rat model by gavage, randomly divided into three groups: normal control group?PQ poisoning group (PQ, 50 mg/kg i.g.; normal saline 50 mg/.kg i.p. qd), ulinastatin therapeutic group (PQ, 50 mg/kg i.g.; Uli, 120000 iu/kg,i.p. Bid). Extracted rat heart tissues on 1st?3rd?7th?14th day respectively for HE and IHC staining, observed the morphological changes of hearts and the expression of NF-kB?ICAM-1?TNF-α, assayed the gray level of TNF-α in tissues, made statistical analysis and explored the significance.Results-HE staining showed that PQ poisoning resulted in rat myocardial vascular dilatation, interstitial congestion and edema, and even myofiber fracture, spot and patchy infarction, worse over time and alleviated until the 14th day; in ulinastatin group, myocardial injury was reduced significantly. Statistical analysis on IHC staining revealed that the expression of NF-kB?ICAM-1 and TNF-α was increased soon after poisoning the rats; in ulinastatin group, higher than that in control group while lower than in PQ group.Conclusion–PQ poisoning can damage rat heart, and the expression of NF-kB?ICAM-1 and TNF-α plays a significant role in PQ caused heart injury. Ulinastatin injection can inhibit the expression of NF-kB?ICAM-1 and TNF-αsignificantly and has a great therapeutic impact on PQ poisoned heart.