118 DIABETES MELLITUS (DM)-INDUCED HYPOSENSITIVE BLADDER IS ASSOCIATED WITH INCREASED INFLAMMATION, APOPTOSIS, EXPRESSION OF EP RECEPTORS, AND URINE NGF IN STREPTOZOTOCIN RAT MODEL

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research (II)1 Apr 2013118 DIABETES MELLITUS (DM)-INDUCED HYPOSENSITIVE BLADDER IS ASSOCIATED WITH INCREASED INFLAMMATION, APOPTOSIS, EXPRESSION OF EP RECEPTORS, AND URINE NGF IN STREPTOZOTOCIN RAT MODEL Yao-Chi Chuang, Wei-Chia Lee, Naoki Yoshimura, Chao-Cheng Huang, and Michael Chancellor Yao-Chi ChuangYao-Chi Chuang Kaohsiung, Taiwan More articles by this author , Wei-Chia LeeWei-Chia Lee Kaohsiung, Macedonia More articles by this author , Naoki YoshimuraNaoki Yoshimura Pittsburgh, PA More articles by this author , Chao-Cheng HuangChao-Cheng Huang Kaohsiung, Taiwan More articles by this author , and Michael ChancellorMichael Chancellor Royal Oat, MI More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1497AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Prostaglandin E2 (PGE2) activating EP receptors and NGF modulate voiding function in different pathological conditions. We hereby investigate the immunohistopathological change and the expression of NGF and four subtypes of EP receptors in streptozotocin (STZ) induced DM rats. METHODS Female Sprague-Dawley rats received 45 mg/kg streptozocin (STZ; i.v.) to induce DM. Continuous CMGs were performed on control rats, and STZ treated rats at week 4 or 12 under urethane anesthesia. The bladder was then harvested for histology, expression of EP receptors and NGF by western blotting, and detection of apoptosis by TUNNEL staining. In addition, 4-hr urine was collected from all groups for urine PGE2 and NGF assay. RESULTS Diabetes induced progressive increase of bladder weight, urine production, intercontraction interval (2.1 fold and 2.6 fold increase at week 4 and 12, respectively), and residual urine in a time dependent fashion. DM induced increased inflammatory reaction, apoptosis mainly at the uroepithelium (9.4 fold and 6.3 fold increase at week 4 and 12, respectively), upregulation of EP2 receptors (2.4 fold) at week 4 and EP3 (1.4 fold) and EP4 (1.6 fold) receptors at week 12, and downregulation of bladder NGF expression (60.6% decrease) at week 12. However, STZ induced increase of urine NGF at week 4 (4.6 fold) and 12 (11.9 fold), and had no significant effects on the expression of EP1 receptors, and urine PGE2 (large variability). CONCLUSIONS DM induced hyposensitive bladder and increased inflammatory reaction, apoptosis, expression of EP receptors, and urine NGF, but decreased bladder NGF. Targeting at NGF and EP receptors may be considered for the treatment or prevention of DM hyposensitive bladder. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e47-e48 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yao-Chi Chuang Kaohsiung, Taiwan More articles by this author Wei-Chia Lee Kaohsiung, Macedonia More articles by this author Naoki Yoshimura Pittsburgh, PA More articles by this author Chao-Cheng Huang Kaohsiung, Taiwan More articles by this author Michael Chancellor Royal Oat, MI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...