116 EXPRESSION OF INSULIN-LIKE GROWTH FACTOR 2 RECEPTOR (IGF2R), LEPTIN RECEPTOR (LEPR) AND SERUM- AND GLUCOCORTICOID-REGULATED KINASE 1 (SGK1) mRNA IN HUMAN AND BOVINE PREGNANCIES

Abstract

The objective was to investigate the expression of insulin-like growth factor 2 receptor (IGF2R), leptin receptor (LEPR), and serum- and glucocorticoid-regulated kinase 1 (SGK1) mRNA in human decidua and bovine pregnancies. The IGF2R, LEPR, and SGK1 mRNA were measured by using quantitative real-time PCR in 25 patients with spontaneous abortion, 23 early pregnancy decidua from patients that underwent elective abortion, 8 cloned bovine pregnancies, and 2 normal bovine pregnancies. The expression of LEPR and SGK1 in the human spontaneous abortion group was lower than the early pregnancy (elective abortion) group (P < 0.05) but the expression of IGF2R was increased in the spontaneous abortion group (P < 0.05). The expression of IGF2R, LEPR, and SGK1 mRNA was decreased in the cloned bovine pregnancy group (P < 0.05). Reduced expression of LEPR and SGK1 mRNA in human pregnancies undergoing spontaneous abortion indicates the key role for these transcripts in spontaneous abortion. Low expression of IGF2R, LEPR, and SGK1 in cloned bovine pregnancies may affect endometrial receptivity and contribute to high spontaneous abortion rate in cloned cattle.