115: A comprehensive analysis of the human decidual and placental villous transcriptomes in women with preeclampsia (PE) using next generation sequencing (RNA-seq)

Abstract

In depth understanding of the decidual and placental villous transcriptomes promises to yield insight into the pathogenesis of PE. We performed genome-wide transcriptome RNA sequencing analysis to discover key transcripts and signature transcriptome pathways involved in gene expression of the preeclamptic decidua and placental villi. The placental decidua basalis and villous tissue transcriptomes were evaluated in women with severe PE (sPE), n=5, mean±SD, GA: 30±2wks. For comparison we used placental decidua and villous biopsies of women matched for GA with spontaneous idiopathic preterm birth (iPTB), n=5, GA: 32±1wks, and physiologic pregnancy at term, n=5, GA: 39±1 wks. Deep RNA sequencing was performed on long and short sequence libraries using the Illumina platform. Statistical analysis and pathway mapping were performed using 'DESeq2', 'edgeR', DIANA miRPath and KEGG. sPE was differentiated from term gestation by 17 miRNA and 632 long decidual transcripts. Comparative analysis among the same groups identified 20 miRNAs and 663 long RNA transcripts that were different in the villi. Analysis of the sequence reads of the decidua and villi of sPE vs iPTB identified a number of up- and down- regulated miRNAs linked to a large number of biological processes (Table). By using RNAseq we report novel candidate miRNAs, long RNAs, and pathways that may be instrumental in the transcriptional regulation of decidual and villous gene expression in women with sPE.