Abstract
Pancreatic ductal adenocarcinoma (PDAC) tissue poses a unique challenge for bulk-transcriptomic gene expression, due to their relative stromal density and abundance of inherent ribonuclease enzymes. Spatial transcriptomic (ST) profiling is an established technique to explore the molecular architecture of human cancers. The use of these ST technologies has evolved from their primary development in fresh-frozen tissue, to formalin-fixed paraffin-embedded (FFPE) samples. This has created interest in applying ST to archival PDAC resection specimens.
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