1130P Anchored multiplex PCR-based targeted sequencing for the detection of fusion transcripts in FFPE samples of non-small cell lung cancer patients

Abstract

The increasing number of drugs targeting genomic rearrangements make their detection an important step for cancer management. Fluorescence in-situ hybridization (FISH) is the gold standard for the detection of ALK and ROS1 fusions but multiplexed technologies are needed to allow analysis of several potential targets simultaneously. We have evaluated a targeted RNAseq panel based on Anchored Multiplex PCR (AMP) for the detection of rearrangements in NSCLC patients.