113 TADALAFIL IMPROVES HYPOXIA, STRUCTURAL DETRUSOR CHANGES AND BLADDER COMPLIANCE AFTER CAVERNOUS NERVE INJURY

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research (II)1 Apr 2013113 TADALAFIL IMPROVES HYPOXIA, STRUCTURAL DETRUSOR CHANGES AND BLADDER COMPLIANCE AFTER CAVERNOUS NERVE INJURY Giorgio Gandaglia, Maarten Albersen, Roberta Buono, Alessandro Nini, Fabio Castiglione, Giorgia Colciago, Fabio Benigni, Alberto Briganti, Francesco Montorsi, and Petter Hedlund Giorgio GandagliaGiorgio Gandaglia Milan, Italy More articles by this author , Maarten AlbersenMaarten Albersen Leuven, Belgium More articles by this author , Roberta BuonoRoberta Buono Milan, Italy More articles by this author , Alessandro NiniAlessandro Nini Milan, Italy More articles by this author , Fabio CastiglioneFabio Castiglione Milan, Italy More articles by this author , Giorgia ColciagoGiorgia Colciago Milan, Italy More articles by this author , Fabio BenigniFabio Benigni Milan, Italy More articles by this author , Alberto BrigantiAlberto Briganti Milan, Italy More articles by this author , Francesco MontorsiFrancesco Montorsi Milan, Italy More articles by this author , and Petter HedlundPetter Hedlund Milan, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1492AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Erectile dysfunction (ED) and lower urinary tract dysfunction are common after radical prostatectomy (RP). Phosphodiesterase 5 (PDE5) inhibitors are used for post-RP ED and may improve continence recovery after nerve-sparing RP. Information is scarce on detrusor integrity or bladder overactivity (BO) in NS-RP. We therefore studied the effect of tadalafil treatment on bladder function and morphology in rats with cavernous nerve-injury (CNI). METHODS After ethical approval, 21 Sprague Dawley rats underwent CNI. Two-weeks treatment with tadalafil (n=7) was started 2 days prior to CNI. After treatment, cystometries were performed in awake rats. Vehicle-treated (n=7) and sham-operated rats (n=5) were controls. Bladder tissues were harvested for organbath studies and immunofluorescence. Values are mean±SEM. RESULTS Body and bladder weights were similar in all rats. Sham rats had regular micturitions and no non-voiding contractions (NVC). 4 out of 7 (p<0.05) vehicle-treated rats and 2 out of 7 tadalafilü-treated rats had NVC. 2 out of 7 vehicleü-treated rats had incontinence episodes but sham or tadalafilü-treated rats did not. No differences in basal and threshold pressures, micturition intervals or volumes, or bladder capacity were recorded between groups. Micturition pressure amounted to 50±10, 45 ±18, and 25±5 cmH2O for sham, vehicle, and tadalafil (p<0.05). Tadalafil-treated rats had no residual volume (RV) whereas sham and vehicle-treated rats had RV of 0.002 ±0.0008 and 0.03 ±0.005ml. Compliance was 0.06 ±0.01, 0.04 ±0.01 (p<0.05), and 0.1 ±0.02 ml/cmH2O for sham, vehicle-, and tadalafil-treated rats. Nerve-mediated contractions amounted to 48 ±18 (sham), 29 ±6 (tadalafil) and 130±38 % of K+ (vehicle; p<0.05). Emax to carbachol amounted to 96 ±18 (sham), 125 ±25 (tadalafil), and 244 ±69 % of K+ (vehicle; p<0.05). Hypoxia was noted in the suburothelium with hydroxyprobe intenstities of 0.00016 ±0.00003 (sham), 0.00065 ±0.00003 (vehicle; p<0.05), and 0.00039 ±0.00006 (tadalafil). The actin/collagen 3 ratio was 0.4 ±0.08 (sham), 1.53 ±0.4 (vehicle; p<0.05), and 0.8 ±0.1 (tadalafil). Compared to vehicle, detrusor VAChT and CGRP nerves were reduced in CNI rats but were more frequent in tadalafil-treated rats compared to vehicle. CONCLUSIONS CNI induced a BO with NVC without increased frequency. Detrusor from CNI rats had muscarinic supersensitivity, hypoxia and altered actin/collagen ratio. These changes were partially counteracted by 2-weeks daily treatment with tadalafil. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e45-e46 Peer Review Report Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Giorgio Gandaglia Milan, Italy More articles by this author Maarten Albersen Leuven, Belgium More articles by this author Roberta Buono Milan, Italy More articles by this author Alessandro Nini Milan, Italy More articles by this author Fabio Castiglione Milan, Italy More articles by this author Giorgia Colciago Milan, Italy More articles by this author Fabio Benigni Milan, Italy More articles by this author Alberto Briganti Milan, Italy More articles by this author Francesco Montorsi Milan, Italy More articles by this author Petter Hedlund Milan, Italy More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...