113 GENOMIC PROFILING OF RENAL CELL CARCINOMA IN PATIENTS WITH END-STAGE RENAL DISEASE

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You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011113 GENOMIC PROFILING OF RENAL CELL CARCINOMA IN PATIENTS WITH END-STAGE RENAL DISEASE Toru Inoue, Keiko Matsuura, Taichiro Yoshimoto, Takahiro Narimatsu, Fuminori Sato, Yoji Nagashima, Shingo Hatakeyama, Chikara Ohyama, Tomonori Habuchi, Masayuki Nakagawa, Masatsugu Moriyama, and Hiromitsu Mimata Toru InoueToru Inoue Oita, Japan More articles by this author , Keiko MatsuuraKeiko Matsuura Oita, Japan More articles by this author , Taichiro YoshimotoTaichiro Yoshimoto Tokyo, Japan More articles by this author , Takahiro NarimatsuTakahiro Narimatsu Oita, Japan More articles by this author , Fuminori SatoFuminori Sato Oita, Japan More articles by this author , Yoji NagashimaYoji Nagashima Yokohama, Japan More articles by this author , Shingo HatakeyamaShingo Hatakeyama Hirosaki, Japan More articles by this author , Chikara OhyamaChikara Ohyama Hirosaki, Japan More articles by this author , Tomonori HabuchiTomonori Habuchi Akita, Japan More articles by this author , Masayuki NakagawaMasayuki Nakagawa Kagoshima, Japan More articles by this author , Masatsugu MoriyamaMasatsugu Moriyama Oita, Japan More articles by this author , and Hiromitsu MimataHiromitsu Mimata Oita, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.179AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Patients with end-stage renal disease (ESRD) show an increased incidence of renal cell carcinoma (RCC) compared with the general population. RCCs in patients with ESRD (RCC-ESRD) have a wide variety of histological subtypes. The copy number aberrations (CNAs) of these RCC-ESRD have not been fully investigated. Therefore, we performed array comparative genomic hybridization (array CGH) to determine the CNAs of RCC-ESRD. METHODS A total of 65 patients with RCCs in ESRD were analyzed. Genomic DNA was extracted selectively from formalin-fixed, paraffin-embedded tissues using laser microdissection. High resolution array CGH was performed using Agilent Whole Human Genome 4x44K Oligo Micro Array Kit. RESULTS We analyzed 65 cases of RCC in ESRD (32 clear cell RCCs, 9 papillary RCCs, 17 ACD-associated RCCs, 3 clear cell-papillary RCCs, 1 chromophobe RCC, 1 MTSCC, 2 unclassified RCCs) by array CGH. Unsupervised hierarchical cluster analysis revealed that RCC-ESRD could be subclassified into two main groups (Cluster A and B). The cluster A contained mainly papillary RCC, ACD-associated RCC, clear cell-papillary RCC. These 3 histologic subtypes did not constitute any independent cluster, suggesting that they shared similar CNAs. On the other hand, the cluster B mainly contained clear cell RCC. Next, we analyzed the patterns of CNAs on each cluster and found that the cluster A showed similar CNA patterns with sporadic papillary RCC, such as 7pq gain and 16 pq gain, suggesting that, as a whole, CNAs of papillary RCC-ESRD resemble those of sporadic papillary RCC. On the other hand, the cluster B had similar CNA patterns with sporadic clear cell RCC including 3p, 14q losses, and 5q gain. Next, we compared the CNAs of papillary RCC, ACD-associated RCC and clear cell-papillary RCC, all of which were categorized in the cluster A. Interestingly, we found that all the three histologic subtypes had similar patterns of CNAs, which resemble those of sporadic papillary RCC, suggesting that they shared similar molecular mechanisms of the carcinogenesis of these tumors, although their histologic phenotypes were clearly different. Moreover, duration of dialysis in cluster A was significantly longer than that of cluster B (p=0.015). CONCLUSIONS RCCs-ESRD can be classified into two categories based on their CNAs. Papillary RCC-ESRD, ACD-associated RCC, and clear cell-papillary RCC may share similar oncogenic mechanism. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e48 Peer Review Report Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Toru Inoue Oita, Japan More articles by this author Keiko Matsuura Oita, Japan More articles by this author Taichiro Yoshimoto Tokyo, Japan More articles by this author Takahiro Narimatsu Oita, Japan More articles by this author Fuminori Sato Oita, Japan More articles by this author Yoji Nagashima Yokohama, Japan More articles by this author Shingo Hatakeyama Hirosaki, Japan More articles by this author Chikara Ohyama Hirosaki, Japan More articles by this author Tomonori Habuchi Akita, Japan More articles by this author Masayuki Nakagawa Kagoshima, Japan More articles by this author Masatsugu Moriyama Oita, Japan More articles by this author Hiromitsu Mimata Oita, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...