1114 THE EFFECT OF ETHANOL INTAKE ON RHO-KINASE SIGNALING PATHWAY IN TYPE 2 DM RATS

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research1 Apr 20111114 THE EFFECT OF ETHANOL INTAKE ON RHO-KINASE SIGNALING PATHWAY IN TYPE 2 DM RATS Soo Jin Kim, Woong Jin Bae, Jin Hee Han, Sung Hoo Hong, Sae Woong Kim, Tae-Kon Hwang, Dae Jin Kim, and Ji Youl Lee Soo Jin KimSoo Jin Kim Seoul, Korea, Republic of More articles by this author , Woong Jin BaeWoong Jin Bae Seoul, Korea, Republic of More articles by this author , Jin Hee HanJin Hee Han Seoul, Korea, Republic of More articles by this author , Sung Hoo HongSung Hoo Hong Seoul, Korea, Republic of More articles by this author , Sae Woong KimSae Woong Kim Seoul, Korea, Republic of More articles by this author , Tae-Kon HwangTae-Kon Hwang Seoul, Korea, Republic of More articles by this author , Dae Jin KimDae Jin Kim Seoul, Korea, Republic of More articles by this author , and Ji Youl LeeJi Youl Lee Seoul, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.711AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Cavernosal smooth muscle relaxation plays a major role in erectile function, which is regulated by the Rho-Kinase signaling pathway and NO/cGMP pathways. Therefore we investigated the effect of ethanol intake on Rho kinase signal pathway in corpus cavernosum of type 2 DM rat. METHODS Otsuka Long Evans Tokushima Fatty (OLETF) rats (n=28) and Long Evans Tokushima Otsuka (LETO, n=28) rats were used. LETO is a normal control of OLETF. The animals were assigned to 4 groups: LC group, LETO rats were fed with isocaloric diets (n=14); LE group, LETO rats were fed with 36 % ethanol, 7g/kg/day for 10 weeks (n=14); OC group, OLETF rats were fed with isocaloric diets (n=14); OE group, OLETF rats were fed with 36 % ethanol, 7g/kg/day for 10 weeks (n=14). During the experiment, body weights and fasting glucose levels were check in all animals. After 10 weeks, RT-PCR for 3 RhoGEFs (PDZ-RhoGEF, LARG, p115-RhoGEF) and western blot for RhoA, Rho Kinase-¥á, Rho Kinase-¥â were performed in corpus cavernosum. The expression of endothelial NO synthase (eNOS) and nervous NO synthase (nNOS) in corpus cavernosum were evaluated by immunohistochemistry. RESULTS In non-alcoholic group, RhoGEFs (PDZ-RhoGEF, LARG, p115-RhoGEF) gene and RhoA/Rho-kinase protein expression in OLETF rats were significantly increased compared to LETO rats (P <0.05). After alcohol oral administration, the increased RhoGEFs/RhoA/Rho-Kinase expression in OLETF rats was obviously decreased (P <0.05). The expression of eNOS and nNOS in corpus cavernosum of alcoholic group were higher than non-alcoholic group. The expression of eNOS and nNOS were decreased in OC group compared with LC, LE and OE groups. CONCLUSIONS This study suggested oral administration of ethanol down-regulated Rho-Kinase signal pathway in corpus cavernosum of type 2 DM rat. These results showed that ethanol intake may have positive effect on erectile function in DM rat through Rho kinase pathway. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e448 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Soo Jin Kim Seoul, Korea, Republic of More articles by this author Woong Jin Bae Seoul, Korea, Republic of More articles by this author Jin Hee Han Seoul, Korea, Republic of More articles by this author Sung Hoo Hong Seoul, Korea, Republic of More articles by this author Sae Woong Kim Seoul, Korea, Republic of More articles by this author Tae-Kon Hwang Seoul, Korea, Republic of More articles by this author Dae Jin Kim Seoul, Korea, Republic of More articles by this author Ji Youl Lee Seoul, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...