111 SKELETAL MYOGENIC DIFFERENTIATION OF URINE-DERIVED STEM CELLS AND ANGIOGENESIS USING MICROBEADS LOADED WITH GROWTH FACTORS FOR POTENTIAL TREATMENT OF STRESS URINARY INCONTINENCE

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research (II)1 Apr 2013111 SKELETAL MYOGENIC DIFFERENTIATION OF URINE-DERIVED STEM CELLS AND ANGIOGENESIS USING MICROBEADS LOADED WITH GROWTH FACTORS FOR POTENTIAL TREATMENT OF STRESS URINARY INCONTINENCE Guihua Liu, Rajesh Pareta, Rongpei Wu, Yingai Shi, Xiaobo Zhou, Hong Liu, Chunhua Deng, Xiangzhou Sun, Anthony Atala, Emmanuel Opara, and Yuanyuan Zhang Guihua LiuGuihua Liu Winston Salem, NC More articles by this author , Rajesh ParetaRajesh Pareta Winston Salem, NC More articles by this author , Rongpei WuRongpei Wu Winston Salem, NC More articles by this author , Yingai ShiYingai Shi Winston Salem, NC More articles by this author , Xiaobo ZhouXiaobo Zhou Houston, TX More articles by this author , Hong LiuHong Liu Norman, NC More articles by this author , Chunhua DengChunhua Deng Guangzhou, China, People's Republic of More articles by this author , Xiangzhou SunXiangzhou Sun Guangzhou, China, People's Republic of More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , Emmanuel OparaEmmanuel Opara Winston Salem, NC More articles by this author , and Yuanyuan ZhangYuanyuan Zhang Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1490AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We showed that USCs display myogenic and endothelial differentiation capacity when cultured in media containing associated growth factors. The goal of this study is to determine whether skeletal myogenic, anigogenic, and neurogenic growth factors released from alginate microbeads can induce USCs to give rise to a skeletal myogenic lineage, improve revascularization and innervation, and recruit resident cells to participate in tissue repair for stress urinary incontinence. METHODS To provide site-specific delivery and targeted release of growth factors to implanted urine-derived stem cells (USCs), we prepared alginate microbeads containing six growth factors. The growth factors were VEGF, IGF-1, FGF-1, PDGF-BB, HGF, and NGF. Radiolabeled growth factors were loaded separately and used to access in vitro release from the microbeads with a gamma counter over 4 weeks. USCs and microbeads were mixed with collagen gel type 1 (2 mg/ml) and subcutaneously injected into nude mice for 4 weeks. RESULTS The imbedded growth factors were released quickly in the first few days of incubation followed by a steady rate of release for a month. Moreover, in vitro endothelial differentiation of USCs by VEGF released from the microbeads confirmed that growth factors released from the microbeads were bioactive. Grafted cell survival was improved and more cells expressed myogenic (MyoD, myf-5, Myosin) and endothelial cell (CD31 and vWF) transcripts and markers compared to controls. More vessel formation and innervation was observed in USCs with growth factors incorporated into microbeads compared to controls. CONCLUSIONS A synergistic mixture of growth factors were released efficiently in a controlled manner from alginate microbeads, thus guiding USCs to cell differentiation and recruiting resident cells to participate in angiogenesis and innervation. This method could enhance tissue regeneration for potential use in cell therapy for stress urinary incontinence. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e45 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Guihua Liu Winston Salem, NC More articles by this author Rajesh Pareta Winston Salem, NC More articles by this author Rongpei Wu Winston Salem, NC More articles by this author Yingai Shi Winston Salem, NC More articles by this author Xiaobo Zhou Houston, TX More articles by this author Hong Liu Norman, NC More articles by this author Chunhua Deng Guangzhou, China, People's Republic of More articles by this author Xiangzhou Sun Guangzhou, China, People's Republic of More articles by this author Anthony Atala Winston Salem, NC More articles by this author Emmanuel Opara Winston Salem, NC More articles by this author Yuanyuan Zhang Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...