Abstract
Publisher Summary This chapter focuses on the purification of yeast artificial chromosomes (YAC) DNA for the production of transgenic mice by microinjection of fertilized oocytes and the analysis of YAC transgene structure in mice. The chapter outlines the manipulations of YACs in yeast. Generation of transgenic mice with YACs and other large molecules has proved to be a valuable system for studies of gene structure-function relationships, production of mouse models of human disease, complementation of mouse mutants, use of mice as bioreactors, and synthesis of in vivo YAC libraries. Continued refinements of current techniques and development of new protocols should encourage widespread adaptation of this strategy for the aforementioned applications. Use of whole loci as transgenes is an important improvement as the advent of murine transgenesis, one that may allow a more realistic assessment of gene regulatory mechanisms during development.
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