Abstract

This chapter discusses the role of transposable genetic elements in bacteria and in maize. Transposable DNA elements can be transposed to new locations, and they can be excised from these locations, restoring the DNA sequence present before they had been inserted. It could be assumed that transposition of an IS element to a new location is accompanied by its loss from the former integration site. There is, however, increasing evidence that the transposition mechanism is more complicated. The techniques that become available in the molecular biology of eukaryotes will allow the isolation of the maize elements. Mutants are available that are caused by insertion of Ds at or in the vicinity of genes that encode known proteins. Of these, gene Sh, encoding endosperm sucrose synthetase, is expressed in endosperm only. The controlling elements could then be isolated as DNA inserted in or adjacent to the gene in the appropriate mutants and it should be absent in this position from the wild type.

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