Abstract
A growth factor derived from bovine cartilage is described which stimulates DNA synthesis and cell division in cultured fibroblasts and chondrocytes. This growth factor is obtained by extraction of bovine scapular cartilage from 1-14 day old calves with 1M guanidine hydrochloride. Further purification is achieved by ion exchange chromatography using a cationic ion exchange resin. The active fraction is eluted with 0.02M ammonium acetate, pH 8.5. This fraction stimulates DNA synthesis in mouse Balb/c-3T3 cells-and NIH/3T3 cells. It also stimulates DNA synthesis in confluent, resting chondrocytes cultured from scapular cartilage of adult New Zealand white rabbits. The growth factor is inactivated by trypsin. On gel filtration in 4M guanidine hydrochloride the growth factor chromatographs in the region of cytochrome c, indicating a molecular weight of 12,000-13,000 daltons. The occurrence of both growth factor and chondrocytes in the same tissue suggests a possible physiological role for this factor in the regulation of cartilage growth.
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