Abstract
Publisher Summary This chapter discusses solid-state nuclear magnetic resonance (NMR) of DNA. Solid-state NMR is one of the most useful techniques, for probing the structure and dynamics of DNA. Since it can measure the orientational as well as distance information, it is a useful complement to liquid-state NMR and the crystallographic methods. The problem, until now, has been resolution. Even highly oriented DNA samples are poorly ordered, by comparison with, for example, single crystals; therefore, if several spin species are present in the sample, it will be difficult to distinguish the contributions from the individual species. For this reason, most solid-state NMR studies of DNA have concentrated on phosphorus, sodium, or deuterium; there is formally only a single phosphorus and sodium species in DNA, whereas deuterium can be selectively attached to the C-8 carbon of purines. Two other variables affect the properties of DNA; excess salt content and humidity. Some excess salt is almost always present in DNA prepared from electrolyte solutions; its quantity can be controlled by modifying the ionic strength of the solution, from which the DNA is precipitated or in which it is bathed after precipitation.
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