11] Ion-exchange chromatography of aminoglycoside antibiotics

Abstract

This chapter discusses the ion-exchange chromatography of aminoglycoside antibiotics. Aminoglycoside antibiotics produced by Streptomyces, Micromonospora, and Bacillus have been extracted and purified by application of an ion-exchange chromatography. More than one aminoglycoside antibiotic is generally produced by the same strain as in cases of kanamycins A, B, and C by Streptomyces kanamyceticus, 1 gentamicins C 1 , C 2 , and C 1a by Micromonospora purpurea and butirosins A and B by Bacillus circulans. Therefore, complete separation of the analogous antibiotics that are produced in a same culture filtrate has been studied. These antibiotics are adsorbed by cation exchangers such as resin, cellulose, and Sephadex ion-exchangers, and separated. Commercially available cation exchangers used for their separation are shown. It is suggested that the most efficient chromatography for separation of analogous aminoglycoside antibiotics can be accomplished by the use of anion exchange resin, Dowex l-X2. This technique by nonionic adsorption chromatography was first employed for separation of kanamycins. Many of the other antibiotics can be purified by this technique.