Abstract
Publisher Summary This chapter describes the electrophysiological expression of ion channels in Xenopus oocytes. The use of the Xenopus oocyte translation system for transient expression has proved to be a critical tool in the molecular characterization of ion channels. The translation of exogenous mRNA in oocytes has been a central component in studies to identify, characterize, and undertake structure-function studies with mRNA species encoding voltage-sensitive ion channels and receptors. This system has been used to confirm the identify of cDNAs encoding voltage-dependent ion channels and numerous receptors. The oocyte has been used in several cases for expression cloning of channels and receptors for which no probe was available. The Xenopus oocyte has been used extensively as a translation system for microinjected mRNA since its introduction. This chapter describes the preparation of suitable mRNA, injection of RNA into oocytes, and recording of new currents. It also describes the application of this system to the study of ion channel regulation in the uterus.
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