Abstract
This chapter discusses the preparation of hybridoma cell lines, which secrete monoclonal antibodies cell surface antigens from two gram-positive bacteria, Streptococcus mutans and Actinomyces viscosus, associated with dental caries formation. A. viscosus monoclonal antibodies were prepared against fimbriae found on the surface of virulent strains of this bacterium. The antibodies were specific for the type 1 fimbriae that mediate binding of the cells to the tooth surface and type 2 fimbriae that promote the cell–cell binding of this bacterium to oral streptococci. Hybridoma cell lines which make monoclonal antibodies to species-specific and serospecific antigens of Actinobacillus actinomycetemcomitans, the primary etiological agent in localized juvenile periodontitis were also produced.
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