1098 Evaluation of Lipid Profile in Cord Blood of Term Newborns in Our Center

Abstract

Background and aims: Clinical studies have demonstrated a close association between lipoprotein abnormalities and susceptibility to cardiovascular diseases. Recent studies have shown that cardiovascular diseases start in childhood. Evaluation of newborns lipid profile might be benefit. So we measured lipid levels in cord blood of newborns. Methods: During August and October 2009, Samples of umbilical cord blood was collected from 200 full term newborns (89 males and 111 females) to be used to determine lipid profile levels: The analysis was performed using Selectra 2 auto analyzer (vital scientific, spankeren, Netherlands). Total cholesterol (TC) and triglycerides(TG) levels were assayed with a sensitivity of 5 mg/dl using enzymatic colorimetric tests with cholesterol esterase and cholesterol oxidase and glycerol phosphateoxidase respectively (ParsAzmon kits, Iran). High-density lipoprotein cholesterol (HDL-c) was measured after precipitation of the Apo lipoproteins with phosphotungistic acid. Low density lipoprotein cholesterol (LDL-c) was calculated from serum TC, TG and HDL-c using Fried Wald formula. It was not calculated when TG concentrations were more than 400 mg/dl. and Non- HDL cholesterol was calculated. Results: Mean TC: 74.58, Mean TGs: 96.59, Mean HDL-C:28.68, Mean LDL-C: 29.25 and Mean Non- HDL cholesterol: 45.90. Biochemical factors which been studied had not significantly difference between genders. Conclusions: Our findings show that cord blood TGs level in neonates of our center are higher than other countries that have been studied previously, no significant difference be observed in others biochemical factors. More detail researches of predisposing factors of cardiovascular system in Iranian population is highly suggested.