Abstract
This chapter discusses the vitamin A storing cell. The localization of vitamin A in animal tissues can be investigated histochemically by various means. Trichloroacetic acid (CCI 3 -COOH), arsenic trichloride (AsCI 3 ), antimony trichloride (SbCI 3 ), and H 2 SO 4 can be used as specific reagents for coloration of vitamin A. Localization of vitamin A is shown by its deep blue color. However, the coloration rapidly fades away within a few hours. The tissues suffer severe damage from the reagents, and the vitamin is dissolved in the lipid-soluble reagents causing difficulty in determining the exact localization of the vitamin within the tissue. Studies performed using antimony trichloride methods revealed that vitamin A is contained in mitochondria. The more sensitive and reliable method to detect vitamin A in situ histochemically employs a fluorescence microscopy. Popper and his associates made extensive studies on the vitamin A containing cells in the body.
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