1087 MALE CHRONIC PELVIC PAIN IS ASSOCIATED WITH THE PRESENCE OF IFN-ψ –EXPRESSING CD4+ T CELLS IN THE PROSTATE

Abstract

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 20121087 MALE CHRONIC PELVIC PAIN IS ASSOCIATED WITH THE PRESENCE OF IFN-ψ –EXPRESSING CD4+ T CELLS IN THE PROSTATE Marsha Quick, Soumi Mukherjee, Anthony Schaeffer, and Praveen Thumbikat Marsha QuickMarsha Quick Chicago, IL More articles by this author , Soumi MukherjeeSoumi Mukherjee Chicago, IL More articles by this author , Anthony SchaefferAnthony Schaeffer Chicago, IL More articles by this author , and Praveen ThumbikatPraveen Thumbikat Chicago, IL More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1194AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Pain is the hallmark of patients with chronic prostatitis (CP) and chronic pelvic pain syndrome (CPPS). The etiology and pathogenesis of this disease syndrome remains unknown. We hypothesized that CD4+ T cells and expressed cytokines play a role in the pathogenesis of pelvic pain. We examined the association of prostate-derived CD4+ T cells and elicited cytokines with the presence of pelvic pain in patients with CPPS and corresponding controls. The role of CD4+ T cells and effector cytokines of the Th1/Th2/Th17 helper T cell lineage in the pathogenesis of pelvic pain was further examined in a murine microbial–induced autoimmune model of chronic pelvic pain. METHODS CD4+ T cells from post-prostatic massage urine of patients with CPPS or controls were functionally phenotyped using intracellular cytokine staining followed by flow cytometry. Expression of IFN-γ, IL-17 and IL-4 were examined for correlation with the chronic prostatitis symptom index and pain subscore using the spearman test. The microbial-induced autoimmune prostatitis model was initiated in NOD/ShiLtJ (NOD) and C57BL/6 (B6) mice using intra-urethral instillation of a clinical Escherichia coli isolate named CP1 from a patient with chronic pelvic pain. Referred hyperalgesia and tactile allodynia were tested using von Frey filaments applied to the abdomen. CD4+ T cells from peripheral lymph nodes and enzymatically-dissociated prostates were phenotyped for the expression of IFN-γ, IL-17 and IL-4. RESULTS In post-prostatic massage urines we observed a significant correlation between expression levels of the Th1 cytokine IFN-γ and the pain subscore of the chronic prostatitis symptom index (spearman r=0.78, p=0.0279). In contrast, there was no significant association of the pain subscore with the levels of IL-17 and IL-4. In the murine model, CD4+ T cells in peripheral lymph nodes and prostates of CP1-infected NOD mice expressed IFN-γ and IL-17 but not IL-4, consistent with a Th1/Th17 immune signature. In contrast, CP1-infected B6 mice, a murine strain resistant to the development of chronic pelvic pain lacked both IFN-γ and IL-17 expression. CONCLUSIONS Our results suggest that pelvic pain is associated with CD4+T cells in the prostate that exhibit a Th1 immune signature. These results have implications for understanding the pathogenesis of CPPS and in developing targeted immune therapies. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e441 Peer Review Report Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Marsha Quick Chicago, IL More articles by this author Soumi Mukherjee Chicago, IL More articles by this author Anthony Schaeffer Chicago, IL More articles by this author Praveen Thumbikat Chicago, IL More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...