(107) Vitamin B12 and Ketorolac on pain and inflammation in Long Evans rats

Abstract

Effects of vitamin B12 on nociceptive pain, inflammatory pain were used in several experimental studies but comparison of these effect with ketorolac tromethamine (KT) and their combination has not been established. To assess the effects of vitamin B12 and KT against pain and inflammation after single administration in rat models and compare them with the combinations of vitamin B12 with KT. This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, from March 2015 to February 2016. For this, 60 (sixty) Long Evans rats (215±35 gm) of both sexes were divided into control (A, with 5 ml/kg normal saline) and experimental (B1, with 15 mg/kg; B2, with 10 mg/kg KT; B3, with B12+KT) groups with 15 rats in each group. All the drugs and vitamin were administered intraperitoneally in a single dose just one hour before the pain evaluation test (tail immersion, formalin and writhing test) followed by formalin induced paw edema test. Afterwards, all rats were deeply anesthetized with chloral hydrate followed by euthanasia. Statistical analysis was done by ANOVA, followed by Bonferroni post hoc test. In the interpretation of results, p≤0.05 was considered as significant. Single dose of vitamin B12 lowered (p≤0.001) variables for nociceptive pain and inflammatory pain. Again, combined administration of vitamin B12 and KT lowered all variables for nociceptive and inflammatory pain (p≤0.001) than individual vitamin B12 and KT administration. In addition, statistically significant lowering of paw edema was observed after KT (p≤0.01) and combination of B12 and KT (p≤0.001) in formalin induced paw edema test. It may be concluded that, vitamin B12 possess analgesic as well as anti-inflammatory effects and combination of B12 with KT is more effective than those of their individual administration.