1068 Protective Effect of Complex Lipid Matrix in the Postnatal Diet on Adult Body Fat Accumulation is Dependent on Lipid-Droplet Size

Abstract

We developed an IMF with a complex lipid matrix (CLM) which more closely resembles the physical properties of fat globules in breast milk, i.e. containing large lipid droplets coated with phospholipids. Postnatal exposure to infant milk formula (IMF) with CLM prevented excessive diet-induced fat accumulation in adult mice (Oosting et al., 2010). However, just increasing the lipid droplet size only marginally affected body composition development (Schipper et al., 2010). Hence, we investigated the contribution of the size of the lipid droplets in CLM on body composition development in mice.Postnatally, between 16 and 42 days of age, male C57Bl/6j mice were subjected to a diet containing standard IMF (CTR), CLM with lipid droplet size similar to CTR (CLM-S), or CLM (CLM-L). Subsequently, a moderate Western style diet (WSD, 10 w% fat) was fed until dissection at 98 days of age. Body composition was monitored by dual x-ray absorptiometry at 42, 70 and 98 days of age.Mice on both CLM diets showed less fat accumulation when challenged with the WSD during adulthood compared to the mice on CTR diet. However, the effect was more pronounced in mice on the CLM-L. In conclusion, although both phospholipid coating as well as increased size of lipid droplets in the postnatal diet protect against excessive fat accumulation when challenged with WSD in adulthood, combining both features enhances the beneficial effect significantly.