Abstract
Gluten content, and more importantly its composition expressed through glutenin subunits, has great influence on the rheological and bread-making properties of wheat flour. Total of 168 winter wheat cultivars developed at the Institute of Field and Vegetable Crops, Novi Sad, Serbia in the period 1987-2008 were analysed for high molecular weight glutenin subunits (HMWGS) composition using SDS-PAGE. Presence of twelve different alleles and nineteen different GS combinations was determined. The highest frequency was found for GS N at the locus Glu-A1 (46%), 7+9 (77%) at the locus Glu-B1 and 5+10 (72.7%) at the locus Glu-D1. The most frequent combination was 2*, 7+9, 5+10. Presence of several rare GS with positive effect (13+16 and 15+16) was determined, as well as high uniformity of the genetic material, considering small number of cultivars (4.2%) with different electrophoretic paths. Two modes of glutenin score (Glu-1 score) determination were applied, based on which differences in bread-making quality among individual cultivars can be determined more precisely.
Highlights
Wheat quality is determined by genetic quality potential and environmental factors (Graybosch et al 1996, Mladenov et al 2001)
Summary: Gluten content, and more importantly its composition expressed through glutenin subunits, has great influence on the rheological and bread-making properties of wheat flour
Total of 168 winter wheat cultivars developed at the Institute of Field and Vegetable Crops, Novi Sad, Serbia in the period 1987-2008 were analysed for high molecular weight glutenin subunits (HMWGS) composition using SDS-PAGE
Summary
Wheat quality is determined by genetic quality potential and environmental factors (Graybosch et al 1996, Mladenov et al 2001). HMW-GS composition is conditioned by cultivar ideotype influenced by many factors: historical (Panin 1999), climatic (Graybosch et al 1996, Mladenov et al 2012), D. The origin and the process of individual HMWGS incorporation vary in wide geographical area. This was significantly aided by adaptation to local agroecological conditions and relation of specific subunits to certain stress factors (Dona et al 2005, Al Jorf 2008). The area of northern Europe (Sweden, the Baltic countries) is characterised by the combinations HMW-GS, 2*, 6+8 or 7+9, and 2 + 12 (Johansson et al 1995), and N or 1, 7+9 and 5+10 (Johansson et al 2003), respectively.
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