Abstract
The liberation of histamine and other inflammatory mediators constitute a major component of host defense. HREF is a unique cytokine produced by PHA stim. mononuclear cells (MC) that may be an important mediator of inflammatory responses. Incubation of granulocytes with HREF enhanced subsequent basophil histamine release (BHR) induced by anti-IgE, f-Met peptide, and the Ca ionophore A23187 (124±43%, 139±40%, and 66±29% enhancement). HREF production was dependent on the number of cells cultured, PHA cone., and duration of culture. Conditioned media containing HREF was active after heat (70°C × 30 min), absorption by activated lymphocytes, and thyroglobulin removal of PHA. HREF produced by cord blood MC of 19 newborns produced significantly less enhancement of anti-IgE induced BHR than adult controls (39±30% vs 53±31% enhancement, p<.05). This was not related to impaired PHA responsiveness. Both stim. and unstim. cord MC incorporated greater 3H thymidine than controls (p <.001). T cell enriched (TCE) populations produced greater HREF than unfrac. or T-depleted (TD) populations (TCE 73±32% enhancement; unfrac. 42±20%; TD 43±25%; N=13, p <0.05). Functional cellular immune deficiency in newborns and deficient lymphokine synthesis by cord lymphocytes have been previously reported. HREF is a potentially important determinant of histamine release and reduced capacity for its production may contribute to the relative immunodeficiency of newborns.
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