104-P: IMPACT OF MATCHING OF HLA CLASS I AND CLASS II ALLELES IN THE DONOR AND RECIPIENT ON THE RISK GRAFT-VERSUS-HOST DISEASE AFTER UNRELATED ALLOGENIC HSCT: A SINGLE CENTER EXPERIENCE

Abstract

Aim The aim of our study was to analyze the impact of matching of HLA class I and class II alleles in the donor and recipient on the risk of grades III-IV aGVHD, cGVHD after unrelated allogenic HSCT. Methods In the present study we retrospectively analyzed the outcomes of 399 HSCT. HLA genotyping was made by PCR-SSP,SSO,SBT methods. Statistical analysis was done by SAS Enterprise Guide v.5.1. Fischer’s Exact Test was used for comparisons the risk aGVHD,cGVHD in the HLA-matched and HLA-mismatched groups. Logistic regression model was used for the analysis association between HLA and non-HLA variables and risk aGVHD. Results 290 patients received a 10/10 allele-matched transplant. 80 patients had a 9/10 allele- matched transplant (single mismatch for HLA-A : 27,5%, HLA-B: 23,7%, HLA-C: 32,5%, HLA-DRB1: 6,3%, HLA-DQB1: 10%). 29 patients received an equal and less than 8/10 allele-matched transplant. The risk of grades III-IV aGVHD was influenced by the number of mismatches, individual class I and class II loci disparities in the recipient. Single mismatched recipients had an increased hazard of grades III-IV aGVHD compared with matched recipients (RR =1.98; CI 1.42-2.76; p Conclusions Single HLA-DRB1, HLA-B, HLA-C alleles mismatches are the significant risk factors of grades III-IV aGVHD for our patients cohort. It should be considered at the selection of the donor for carrying out unrelated allogenic HSCT .