Abstract
Publisher Summary This chapter discusses the folic acid conjugase. The folic acid conjugases may be described as a group of enzymes which act on conjugates of folic acid to release substances having “folic acid activity” for Streptococcus faecalis or Lactobacillus casei . These enzymes are distributed quite widely in nature and may be divided into two groups: (1) the type present in chicken pancreas, having a pH optimum of 7.0 to 8.0, and (2) the type widely distributed in animal tissues, especially liver and kidney, with an optimum pH around 4.5. Recent evidence indicates that the folic acid activity of natural materials is due in part to citrovorum factor (CF). When yeast extract, a potent source of folic acid heptaglutamate, is treated with folic acid conjugase, the apparent CF content markedly increases, from which it is inferred that CF, such as PGA, exists in conjugated form. Although the precise mechanism is not known whereby folic acid conjugates are converted to PGA or CF, it is advisable to follow the practice of liberating folic acid activity from its conjugates with crude tissues high in folic acid conjugase. An alternative procedure of the conjugate-splitting enzyme from hog kidney may also be used where desiccated hog kidney or a water extract of fresh hog kidney is used.
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