Abstract
The use of small interfering RNA (siRNA) has become increasingly popular in recent years for gene knockdown both in vivo and in vitro. To date, a majority of expression systems are utilizing a RNA pol III promoter. The pol III promoter, although supporting high levels of siRNA expression, cannot be easily regulated nor engineered to confer tissue specificity. In this study we utilize recombinant adeno associated virus (rAAV) to express siRNA targeting tyrosine hydroxylase (TH), a protein involved in the production of dopamine, using an expression cassette containing the chicken beta-actin (CBA) pol II promoter.
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