Abstract
The mechanisms regulating expression of IgA subclasses are poorly understood. While adult serum contains ˜90% IgAl and ˜10% IgA2, breast milk contains a 40/60 mix respectively. In the present study colostrum was collected from 11 donors (days 1–5 post-partum), the cells washed through a fetal calf serum cushion, separated over ficoll-Hypaque, and twice washed with buffer. The mononuclear cell fraction was assessed by Wright-Giemsa stain and flow cytometry using fluorescein-conjugated monoclonal anti-T4, T8, IgA1 and IgA2 antibodies. A strong correlation was found between the % lymphocytes and % IgA2+ cells (R=.842). The T4/T8 ratio did not correlate, and averaged 1.11+/−.67 No correlation was found with surface IgAl+ cells. The findings are consistent with a model which proposes either recruitment to or expansion of sIgA2+ lymphocytes in colostrum.
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