Abstract
A compact perfusion system with oscillatory flow was developed for bone tissue engineering, and its seeding and culture effects were evaluated in present study. Mouse osteoblast-like cells, MC 3T3-E1, were seeded and cultured for 6 days in porous ceramic scaffolds by either static or oscillatory perfusion method. Compared with static culture, the oscillatory perfusional method showed significantly higher seeding efficiency and higher scaffold cellulary by DNA content analysis, as well as higher ALP activity and after 6 days of culture. Stereomicroscopy observation of MTT staining and Hoechst/PI double staining also demonstrated homogeneous seeding, proliferation and viability spatially in oscillatory system ; on contrast, static culture showed a polarized seeding and proliferation favoring only the outer and upper surface, with cells in central part of scaffold underwent apoptosis. The results demonstrated the novel oscillatory perfusion system could be a simple but useful bioreactor for bone tissue engineering with easier operation.
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