Abstract
A compact perfusion system with oscillatory flow was developed for bone tissue engineering, and its seeding and culture effects were evaluated in present study. Mouse osteoblast-like cells, MC 3T3-E1, were seeded and cultured for 6 days in porous ceramic scaffolds by either static or oscillatory perfusion method. Compared with static culture, the oscillatory perfusional method showed significantly higher seeding efficiency and higher scaffold cellulary by DNA content analysis, as well as higher ALP activity and after 6 days of culture. Stereomicroscopy observation of MTT staining and Hoechst/PI double staining also demonstrated homogeneous seeding, proliferation and viability spatially in oscillatory system ; on contrast, static culture showed a polarized seeding and proliferation favoring only the outer and upper surface, with cells in central part of scaffold underwent apoptosis. The results demonstrated the novel oscillatory perfusion system could be a simple but useful bioreactor for bone tissue engineering with easier operation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
