Abstract
This chapter discusses the glycosylation of rhodopsin. The oligosaccharide of rhodopsin is relatively simple, consisting of two separate chains, each composed of mannosc and N -acetylglucosa-mine. Structures of this type are synthesized on microsomal membranes as lipid-linked oligosaccharides which are transferred en bloc to an asparagine residue of the newly synthesized polypeptide. The transfer of galactose and fucose from the respective sugar nucleotides to rhodopsin can be demonstrated with bovine rod outer segment preparations. The site of this secondary glycosylation of opsin can be demonstrated by the use of another inhibitor in vitro . A large intracellular pool of uridine diphosphate (UDP)-N-acetylglucosamine is inferred from the relatively slow autoradio-graphic labeling of the rough endoplasmic reticulum. The synthesis of lipid-linked oligosaccharide intermediates has been described in chick embryo retina. Comparable oligosaccharide-lipids are formed by cell-free bovine retina preparations with evidence that they are also transferred to rhodopsin.
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