102. Changes in the reactive species interactome in oocyte donation pregnancies and pre-eclampsia

Abstract

Introduction Oocyte donation (OD) is an artificial reproductive technique after which the fetus is completely allogenic to the mother. OD pregnancies carry a higher-than-normal risk to develop pre-eclampsia, which is characterized by an angiogenic imbalance, endothelial dysfunction and oxidative stress. Objective/Hypothesis We sought to investigate oxidative stress status in OD and pre-eclampsia by the measurement of different elements of the reactive species interactome (RSI). We hypothesized that the RSI is affected by OD and pre-eclampsia. Methods Between 2012 and 2016, sera were collected from women with uncomplicated naturally conceived (NC) pregnancies (n = 23), uncomplicated OD pregnancies (n = 27), NC pregnancies complicated by pre-eclampsia (n = 24) and OD pregnancies complicated by preeclampsia (n = 5). Indicators for reactive species production such as free thiols, protein-bound nitric oxide (RxNO), nitrite, nitrate, 8-iso-prostaglandin F2a, sulfate, and thiosulfate were measured using a variety of analytical techniques in serum. Results Nitrite levels were higher in uncomplicated OD pregnancies compared to NC pregnancies (p 0.001) and free thiol levels seem lower in OD compared to NC pregnancies. OD pregnancies complicated by pre-eclampsia resulted in decreased nitrite levels (p = 0.001) and decreased free thiol levels (p 0.001) compared to uncomplicated OD pregnancies. Decreased nitrite (p = 0.01) and free thiol levels (p 0.001) were also seen in NC pregnancies complicated by pre-eclampsia compared to uncomplicated NC pregnancies. RxNO levels(p = 0.005) and sulfate levels (p = 0.001) were increased in NC pregnancies complicated by pre-eclampsia compared to uncomplicated NC pregnancies. Discussion OD pregnancies differ from NC pregnancies with respect to the RSI. Changes in the RSI in uncomplicated OD pregnancies suggest a role for perturbations in redox status in the development of pre-eclampsia in OD. Redox differences between pre-eclampsia after OD and pre-eclampsia after NC pregnancy suggest variations in what triggers their initiation and progression. The RSI might represent a therapeutic opportunities to prevent pre-eclampsia in OD pregnancies.