Abstract
Many vectors are prone to waning transgene expression. To maintain expression one must first understand how inactivation occurs. Although SV40-derived vectors (rSV40s) are not susceptible to waning transgene expression, some transgenes (mainly of nonvertebrate origin, e.g., red fluorescent protein, RFP), express well when virus genomes are transfected but poorly on rSV40 transduction. Studying gene silencing in this system may provide useful insights into regulation of transgene expression.
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