Abstract

Three-dimensional (3D) fluorescence imaging is an essential technique in the biomedical field. In particular, 3D fluorescence imaging through dynamic scattering media is a crucial task for the minimally invasive observation of labeled cells. In this study, this task was accomplished via motionless optical scanning holography, proposed as a single-pixel 3D imaging technique. The proposed method does not require additional computational processing or optical components when the detected intensities do not considerably fluctuate irrespective of the presence of dynamic scattering media. The results of a proof-of-principle experiment indicated that the proposed method can help in computationally refocusing fluorescent objects that are placed at different positions behind dynamic scattering media.

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