Abstract
We examined platelet-dependent thrombin generation in patients with coronary artery disease (CAD). Thrombin generation was measured according to the method of Aronson et al (Circulation, 1992). 0.5 ml of platelet rich plasma (PRP, 15 × 10 4 /ml) was prepared, and 40 mM of CaCl, was added to start clotting. 0.5 mM of S-2238 was added to each sample in a microtiter plate every 10 min, and the plate was read kinetically at a wavelength of 405 nm on a microtiter plate reader. The patients with CAD devided into 3 groups. Thrombin generation 20 min after CaCI 2 , additon is: Control (n = 12) 48 ± 10(mOD) Stable angina (SAP) (n = 15) 79 ± 27 Unstable angina (UAP) (n = 15) ** 562 ± 155 Acute myocardial infarct (AMI) (n = 43) ** 440 ± 269 ** p < 0.01 compared to SAP The patients with UAP and AMI showed marked increase in thrombin generation compared to SAP and control subjects. AMI patients with severe coronary artery disease (Group B) showed higher levels of thrombin generation (Group A, Gensini score < 32: 382 ± 248 mOD vs Group B, Gensini score> 31: 578 ± 238, P < 0.05). LVEF of group A is significantly higher than that of group B (P < 0.05). These findings indicate that patients with UAP and AMI have an evidence of hypercoagulable states and that platelet-dependent thrombin generation may play an important role in pathophysiology of UAP or AMI, and may be a prognostic factor in CAD.
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