重组干扰素诱导蛋白-10对视网膜血管内皮细胞增生、迁移及管腔形成的影响

Abstract

Objective The observe the effects of interferon-inducible protein-10 (IP-10) on proliferation,migration and capillary tube formation of human retinal vascular endothelial cells (HREC) and human umbilical vein endothelial cells (HUVEC).Methods The chemokine receptor (CXCR3) mRNA of HREC and HUVEC were quantified by reverse transcriptase polymerase chain reaction (RT-PCR).In the presence of the different concentrations of IP-10,the difference in proliferation capacity of HREC and HUVEC were analyzed by cell counting kit-8 (CCK-8) methods.Wound scratch assay and three-dimensional in vitro matrigel assay were used for measuring migration and capillary tube formation of HREC and HUVEC,respectively.Results RT-PCR revealed both HREC and HUVEC expressed CXCR3.The proliferation of HREC in the presence of IP-10 was inhibited in a dosage-dependent manner (F=6.202,P＜0.05),while IP-10 showed no effect on the inhibitory rate of proliferation of HUVEC (F=1.183,P＞0.05).Wound scratch assay showed a significant reduction in the migrated distance of HREC and HUVEC under 10 ng/ml or 100 ng/ml IP-10 stimulation (F=25.373,23.858; P＜0.05).There was no effect on the number of intact tubules formed by HREC in the presence of 10 ng/ml or 100 ng/ml IP-10.The number of intact tubules formed by HREC in the presence of 1000 ng/ml IP-10 was remarkably smaller.The difference of number of intact tubules formed by HREC among 10,100,1000 ng/ml 1P-10 and nonintervention group was statistically significant (F=5.359,P＜0.05).Conclusion IP-10 can inhibit the proliferation,migration and capillary tube formation ability of HREC and the migration of HUVEC. Key words: Retinal neovascularization; Chemokine CXCL10/antagonists & inhibitors; Endothelial cells